Author(s):
Wangta Liu, Yow-Ling Shiue, Yi-Reng Lin, Hugo You-Hsien Lin and Shih-Shin LiangPages 300-306 (7)
Abstract:
In this study, we demonstrated an oxidative method with free radical to generate 3,5,4 -trihydroxy-transstilbene (trans-resveratrol) metabolites and detect sequentially by an autosampler coupling with liquid chromatography electrospray ionization tandem mass spectrometer (LC-ESI–MS/MS). In this oxidative method, the free radical initiator, ammonium persulfate (APS), was placed in a sample bottle containing resveratrol to produce oxidative derivatives, and the reaction progress was tracked by autosampler sequencing. Resveratrol, a natural product with purported cancer preventative qualities, produces metabolites including dihydroresveratrol, 3,4 -dihydroxy-trans-stilbene, lunularin, resveratrol monosulfate, and dihydroresveratrol monosulfate by free radical oxidation. Using APS free radical, the concentrations of resveratrol derivatives differ as a function of time. Besides simple, convenient and time- and labor saving, the advantages of free radical oxidative method of its in situ generation of oxidative derivatives followed by LC-ESI–MS/MS can be utilized to evaluate different metabolites in various conditions.
Keywords:
Free radical, metabolite, resveratrol, ammonium persulfate (APS), liquid chromatography tandem mass spectrometry (LC–MS/MS), multiple reaction monitoring (MRM).
Affiliation:
Department of Biotechnology, Kaohsiung Medical University, P.O. Box: 80708 Kaohsiung, Taiwan.
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