A New Method for Measuring Total
Antioxidant Capacity in Urine Using the Iodine Starch Agar Based on Agar
diffusion
Author(s):
Ying Zhou, Naijin Xu, Meijuan Zhang and Hui LiuPages 425-430 (6)
Abstract:
The purpose of this paper is to develop a new
method for determining total antioxidants capacity (TAC) in urine. In our
study, Na2S2O3 and mixed
urine were used to evaluate the linearity and precision of the iodine starch
agar method. The areas of urine diffusion in samples from 44 coronary heart
disease patients and 44 healthy subjects were determined by the iodine starch
agar method. The results have shown that the linearity and precision for iodine
starch agar method were acceptable, because the R2s in linear experiment of Na2S2O3 and urine
were 0.999 and 0.995, respectively; variation coefficients of urine diffusion
area in high and low levels within-run, between-run, between-day were all less
than 10%. The difference of TAC in urine between the coronary heart disease
group (Median= 0.95) and healthy group (Median= 1.13) was statistically
significant (p=0.001). The iodine starch agar method is a simple and convenient
assay to determine total antioxidant capacity in urine.
Keywords:
Iodine starch agar method (ISAM), total antioxidant capacity
(TAC), urine.
Affiliation:
College of Medical Laboratory, Dalian Medical University,
Dalian, 116004, P.R. China.
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Abstract:
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